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The vaginal tissue was placed in a sterile 6-cm culture dish containing 5 mL ice-cold PBS to remove gross adipose, blood clots and vascular tissue, followed by enzymatic digestion in a solution of 1 mg/mL Pronase (10165921001, Sigma-Aldrich, MO, USA) and 0.5 mg/mL DNase I (10104159001, Sigma-Aldrich) under gentle agitation at 4 C for 16 h